Pierluigi Mauri is chief of Proteomics and Metabolomics Labat ITB-CNR. He has a notable experience in the development and application of several technologies (HPLC, capillary electrophoresis, mass spectrometry) for the identification and quantification of several biomolecules in complex matrices. He has been involved in the study of the bioavailabily of several drugs in humans and animals.
He has set-up a proteomics laboratory based on the MudPIT (Multidimensional Protein Identification Technology), a gel-free approch (using two dimensional chromatography coupled to tandem mass spectrometry, 2DC-MS/MS) and a parallel computing system for proteomics. This instrumentation allows for the proteomic profiling of biological samples, without limitations, according to to protein molecular weight, pI or hydrophobicity and guarantees high productivity. Using this methodology, secreted proteins from tumor (FASEB 2005) and immune system cells (JEM, 2011, Immunity, 2016) have been characterized.
Furthermore, direct analysis of biological samples (JMS 2007), enzymatic complexes (Biochimie 2006), development of novel methodological approaches for studying switchredox proteins (JBC 2005 and 2006, JMB 2006), degenerative diseases (Blood, 2012) and structural and functional characterization of proteins (Biol. Chem. 2004, FEBS 2006) have been performed. His main investigations concern molecular profiles obtained by MS-based methodologies and focused on clinical applications (J Proteome Research,
2014; JACC 2013; Nature Commun, 2014; Cardiovasc Res. 2011; Biol Reprod. 2013), including computational methods (J Clin Bioinforma. 2013; Methods Enzymology, 2008) He is co-author of more than 150 scientific publications, he has participated in numerous international congresses and has taught in several metabolomics and proteomics courses.